Soil quality — Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil
文件核验信息(付费前可核对)
标准范围(官方英文摘要)
ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.
It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.
标准信息
- 标准号
- ISO 17601:2016
- 英文标题
- Soil quality — Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil
- 发布单位
- —
- 技术委员会
- ISO/TC 190/SC 4
- 版本 / 版次
- 1
- 状态
- 现行
- 发布日期
- 2016
- ICS 分类
- 13.080.30 土壤的生物特性
- 页数
- 31 页
- 官方定价
- CHF 151
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